CYP450 3A4 / 3A5 Genotyping
Cytochrome P450 3A4/5 (CYP3A4/3A5) acts on approximately half of all drugs in clinical use. About 8% of Caucasians have a slow acting, intermediate metabolizer form of CYP3A4 (CYP3A4 *22). [34]
Prevalence of CYP3A5 variants differs widely by ethnic origin. Approximately 11-12% of Asian and African Americans have non-functional CYP3A5 alleles (CYP3A5 *6, *7). CYP3A4 and CYP3A5 are closely related and may process many of the same drugs. Substrates include opioid pain medications, statins, chemotherapeutic drugs, and combined oral contraceptives.[35]
Genelex offers improved detection rates using a Cytochrome P450 3A4 mutation panel and a Cytochrome P450 3A5 DNA test. These tests each identify common clinically relevant alleles in their respective genes (CYP3A4: *1B,*22. CYP3A5: *3,*6,*7) in PCR-multiplex format. Analytical specificity and sensitivity for detection of these mutations are >99%.
Genelex offers improved detection rates using a Cytochrome P450 3A4 mutation panel and a Cytochrome P450 3A5 DNA test. These tests each identify one clinically-relevant single nucleotide variant in their respective genes in PCR-multiplex format. Analytical specificity and sensitivity for detection of these mutations are >99%.
Indication for CYP3A4/CYP3A5 Testing
For individuals with a personal or family history of adverse drug reactions to medications metabolized by CYP3A4/3A5. In these cases, it's critical to confirm the presence of genotypes that affect the metabolism of drugs such as oxycodone (OxyContin®), simvastatin (Zocor®), or tacrolimus (Prograf®) that are metabolized by cytochrome CYP3A4/3A5.
Insurance Coverage
Check with your insurance provider to determine if CYP3A4/3A5 genotyping is covered. CPT Codes: CYP3A4 and CYP3A5 Mutation DNA Analysis: 81230; 81231 (provided as guidance only).
Specimen Information
Please call Client Services at 800-837-8362 to order testing supplies.
- Buccal Swabs: 4 sterile buccal swabs
- Blood: 5-10cc whole blood lavender-top EDTA or yellow-top ACD-A tubes
- Turnaround Time: 10 business days, faster turnaround available for clinical trials
Additional Info
If you would like to learn more about CYP3A4/CYP3A5, click here to view the short webinar from YouScript, Introducing CYP3A4 and CYP3A5 to the DNA sensitivity testing panel.